FastQCFastQC Report
Wed 7 Jan 2015
trsmbk2.pe.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenametrsmbk2.pe.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8300918
Sequences flagged as poor quality0
Sequence length36-293
%GC63

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTAGA8250.0047109425166.1797285-287
TACTAAA4356.6102494E-4157.58421285-287
TTAGCCC12155.460807E-499.671585285-287
AAATAAC21350.00511263156.721767285-287
AGTAAGA5752.7594002E-454.397953270-274
TGCTTAT22900.00674628452.882523285-287
CTCCCTT18000.00897922449.19794275-279
TATTCAA27100.002716827234.174793280-284
ATATGAG23301.7868064E-529.420225285-287
TATGTTT16750.00398589220.410456275-279
CATAAAT13600.007682633618.220058270-274
AGTTAAA9150.00391283916.769005275-279
AGTATCG52700.00521536314.274025280-284
GCTCCCT24459.20243E-414.188545270-274
GTAGCAA19854.1511506E-511.453216255-259
TCCTTTA19200.00889426510.482027260-264
TTTAGGC9556.0130324E-49.145464265-269
Produced by FastQC (version 0.11.2)