FastQCFastQC Report
Fri 2 Jan 2015
trimmed_pe_2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenametrimmed_pe_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9475726
Sequences flagged as poor quality0
Sequence length34-301
%GC63

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTTT9250.0040604183174.66843295
TGCTAGG22153.7674708E-4109.41419295
CCCCACC330350.063.580685295
CAAGGGG53301.610732E-460.626003295
TTTGATT40650.00419624759.619297295
CAATTTT42600.0050515156.890244295
CATTTTT76201.4714489E-553.00797295
CCGGGGG62753.6077487E-451.49587295
CTCCACC159401.6952981E-840.544117295
GCTCTTT101908.1896214E-539.63894295
TTCTCCC86100.001713019737.53038295
TTCTTTT88100.001917492836.678387295
CCCACCC315850.035.80744295
TGGCCCC93500.00256745834.560066295
ATCCACC118251.9657321E-434.158203295
CTCATCC95850.002899731733.712738295
TCCACCC156252.7622962E-531.021114295
GGCCCCC189202.962619E-629.888426295
CCCCCCC2939550.029.405535295
CGCCCCC637000.029.16853295
Produced by FastQC (version 0.11.2)