FastQCFastQC Report
Fri 2 Jan 2015
trimmed_pe_1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenametrimmed_pe_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9475726
Sequences flagged as poor quality0
Sequence length35-301
%GC63

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGGG4900.001999709871.90901295
TTAATTT14501.2744113E-648.600574295
CTACTTT27109.244117E-526.003994295
GTATCTT36607.060543E-419.254326295
AAATCCC51351.77686E-518.298172295
CGGGAGG72906.295522E-817.722431295
CCTGGGG41150.001550285517.125353295
ATGATTT76101.0280928E-716.977205295
CAAGGGG55403.4116274E-516.960487295
CATTTTT84051.744229E-816.768787295
TCTATTT44650.00267492315.782941295
GAGTTTT61508.33781E-515.278229295
TCACTTT54405.702086E-415.113229295
GGTTGGG56457.548397E-414.564388295
TTCTCTT56557.6502835E-414.538632295
ACCATTT64801.3014532E-414.500171295
GTATTTT49700.005455256414.179242295
TGTTTTT92108.957213E-714.027853295
GGGTGGG51250.006686439313.750406295
AAGTGGG60250.001235336413.645804295
Produced by FastQC (version 0.11.2)