FastQCFastQC Report
Sat 3 Jan 2015
trimmed30_pe_2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenametrimmed30_pe_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9475012
Sequences flagged as poor quality0
Sequence length30-301
%GC63

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTTTT52801.9372692E-6408.45453295
TATTTTT47707.861655E-4301.41718295
ACTTTTT52650.0010566517273.07883295
AATTGGG26900.0015133756267.24164295
ATTTTTT67300.0022035597213.63448295
GCTACCC74500.002986933192.98793295
CCCACCC166708.696552E-7172.49669295
CTTTTTT89650.0051967055160.3748295
CGTACCC96550.0064867116148.91351295
ACACCAC96750.006526972148.60568295
CCCCACC182202.7081772E-4118.36663295
TCTAGGT5550.00445881258.736115285-289
TTAGTTT8400.00299619533.507286290-294
ATTAAAT17200.007040520728.136488290-294
CCCCCCC1521150.001629231723.629492295
TCTTAAC12700.003408969615.220095285-289
ATTTAGA8400.005390483414.2061405280-284
TTAATAA10154.0989296E-413.865084290-294
CTAAGAT6950.001573807410.788447270-274
GATTTAA18000.0094394559.9520445290-294
Produced by FastQC (version 0.11.2)