FastQCFastQC Report
Sat 3 Jan 2015
trimmed30_pe_1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenametrimmed30_pe_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9475012
Sequences flagged as poor quality0
Sequence length30-301
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATTT25650.003470763862.557247295
CAATTTT51602.314282E-762.193542295
CCTGAGG44757.244207E-659.76139295
GACTTTT56304.2276224E-757.00154295
AACATTT39252.7279582E-454.508484295
ACATTTT39552.832497E-454.095016295
GGGTAGG30500.006888623352.609623295
CAGAGGG30550.006933388852.523518295
ATGGGGG33250.0096898848.258446295
CCAGAGG33500.00998088447.89831295
AATTTTT59253.8132974E-545.136242295
TTCCCCC50059.0442796E-442.74641295
CTCATTT51009.921187E-441.950153295
CTGTTTT105001.6274498E-840.75158295
ATGGATT71701.1739509E-437.29878295
CAGTTTT72651.2684982E-436.811047295
TCTTTTT89551.0314556E-535.836815295
GAGTTTT63050.002811989333.932716295
AAGGTTT63750.002968364433.560123295
ATCTCTT63800.002979792433.533825295
Produced by FastQC (version 0.11.2)