FastQCFastQC Report
Fri 2 Jan 2015
smbk2_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamesmbk2_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9475954
Sequences flagged as poor quality0
Sequence length35-301
%GC63

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGG30001.3943827E-716.531427295
ACGGGGG58550.015.400757295
AGGGGGG32954.0463965E-715.051376295
CCCGGGG91750.013.759128295
TAGACCC26352.1246194E-413.688261295
AGAGGGG26552.2652304E-413.585149295
CCCACCC399200.013.552819295
CCCTACC86650.013.528316295
GGTTAGG23650.001462714913.344607295
GGGGGGG61850.013.121144295
CTCCCAC134450.013.078043295
TGTTACC27803.3431937E-412.974306295
CACTCCC132200.012.618543295
CACCCCC384900.012.416433295
CCACCCC415650.012.365623295
CACCTCC166150.012.210997295
AAGGGGG38203.0537012E-511.802543295
GACCCCC151050.011.6407995295
TTACTCC27550.00459529711.455536295
CACACCC193100.011.440704295
Produced by FastQC (version 0.11.2)