FastQCFastQC Report
Fri 2 Jan 2015
smbk2_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamesmbk2_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9475954
Sequences flagged as poor quality0
Sequence length35-301
%GC63

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTTT8956.7451457E-414.783962295
CGGGGGG38900.014.091727295
TGGGGGG17853.49537E-813.766418295
AACCCCC28505.456968E-1212.601567295
AGTCCCC20301.8758692E-712.104955295
TCGGGGG57950.012.068801295
TAAGGGG11600.004745619411.406591295
AAGGGGG29901.8189894E-1011.379343295
GCAGGGG93300.011.142866295
GTGGGGG39250.011.076537295
GAAGGGG78350.010.856487295
CGAGGGG71800.010.793822295
CCCGGGG61600.010.739973295
ACGGGGG41100.010.577958295
TAGAGGG14750.002405316610.252123295
TATAGGG17308.1247033E-49.833594295
GGAGGGG30954.3011823E-89.771813295
CTCAGGG33401.357148E-89.620957295
TACGGGG37901.1041266E-99.476113295
GTAGGGG20303.3721892E-49.311503295
Produced by FastQC (version 0.11.2)