FastQCFastQC Report
Thu 22 Jan 2015
smbk1_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamesmbk1_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20625459
Sequences flagged as poor quality0
Sequence length35-301
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGG90350.046.329586295
TTAGATT24450.043.957157295
TCTCTTT142600.041.650925295
AGGGGGG68800.038.642418295
CTAGATT74650.037.88742295
TCTTATT76400.036.27919295
GGAGGGG100200.036.12993295
TTAGGGG23600.031.159193295
AGAGGGG76900.028.687529295
AAAGGGG101500.027.864986295
GGGGGGG117750.027.862621295
CTAGGGG21306.2718755E-926.55677295
TAGGGGG29150.025.226656295
ATGTGTT182700.024.459267295
AGAGAGG69900.024.27722295
TGGGGGG59650.023.707428295
TAAGGGG30455.0931703E-1022.291988295
AAGGGGG98600.021.226562295
CCTTATT64550.021.03148295
GATCGGG607750.020.197128295
Produced by FastQC (version 0.11.2)