FastQCFastQC Report
Thu 22 Jan 2015
smbk1_R2.t.p.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamesmbk1_R2.t.p.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12020342
Sequences flagged as poor quality0
Sequence length36-281
%GC62

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCTTA115850.066.465904275
TCTGTCT46551.2165765E-763.62121275
TTAATAA6350.00455404758.42126260-264
TGTCTCT138450.055.616283275
CTCTTAT122050.048.53039275
TACACCT40600.00197980243.767002275
ATGTGTA56800.001018792441.71221275
CATCTAG57851.9906405E-540.95512275
ATTATAC8400.001072583435.19782270-274
GTCTCTT151200.034.60868270-274
TTATACA105350.033.734035275
TCTTATA107700.032.969826270-274
CTTATAC132300.031.339334275
ACCTGTC114004.2564352E-1031.174393275
TCCTTAA12100.00446068730.89039270-274
CTGTCTC171150.029.48259270-274
ATCCTTA19655.2200607E-428.935574270-274
TAAATGT10050.0068082328.33114265-269
CCCTGTC66658.44721E-626.660769275
GCTGTCT91300.025.950205275
Produced by FastQC (version 0.11.2)