FastQCFastQC Report
Tue 20 Jan 2015
smbk1_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamesmbk1_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20625459
Sequences flagged as poor quality0
Sequence length35-301
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCTTT140800.073.49568295
CTAGATT37750.070.741745295
TCTTATT63950.059.656002295
ATGTGTT190400.029.679468295
AGAGCAC291150.027.5441638
GATCGGG643800.024.295595295
AAGAGCA349600.022.9791987
GGAAGAG363000.022.1308235
TCGGAAG384000.021.0301653
TAGATGG73600.019.761812295
GAGCACA412650.019.6042799
ATCGGAA477300.017.2724022
CGGAAGA492350.016.7444254
TTAGATT16852.766592E-715.565659295
GATCGGA552650.015.349451
CCTTATT61100.015.21942295
CAGGGGG86150.013.008203295
CTTATAT66950.012.821139295
GTAGATT43650.012.563722295
GAAGAGC663900.012.3543656
Produced by FastQC (version 0.11.2)