FastQCFastQC Report
Wed 21 Jan 2015
smbk1_R1.trimalladapt.fa

Summary

[OK]Basic Statistics

MeasureValue
Filenamesmbk1_R1.trimalladapt.fa
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20221325
Sequences flagged as poor quality0
Sequence length1-301
%GC62

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
C374070.18498787789623083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCTTT97950.0160.70706295
TCTTATT41400.0152.28098295
CTAGATT18150.087.38406295
TATACAC352550.053.534668295
CTTATAT38950.046.827316295
CTCTTAT463850.044.621254295
TGTCTCT530800.038.84376295
GTCTCTT530150.038.741806295
TCTCTTA423950.038.065666290-294
TCTTATA384100.037.99809290-294
TTATACC87250.031.356855295
CTTATAC464650.030.037624295
TCCCTTT61000.029.25038295
CCTGTCT372900.028.815475295
CTGTCTC610550.027.925188295
ATACACC125350.027.836025295
TTATACA362150.026.008509290-294
CTGTCTT90200.024.61673295
ATGTGTT114500.023.201612295
CTCTAGG8550.001901974723.187437295
Produced by FastQC (version 0.11.2)