FastQCFastQC Report
Wed 21 Jan 2015
smbk1_R1.trimall.2.fa

Summary

[OK]Basic Statistics

MeasureValue
Filenamesmbk1_R1.trimall.2.fa
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16930502
Sequences flagged as poor quality0
Sequence length36-281
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT121700.037.6784864
GTCTGAA131100.034.7238549
CACGTCT134550.033.771366
AGCACAC162500.028.473552
ACGTCTG166100.027.3568747
CGTCTGA176450.025.9876028
GAGCACA195650.023.861351
ACACGTC202250.022.9187345
CTCTTAT73950.020.323086275
TCTCTTA71650.016.834738270-274
TCCCTGT37358.074567E-716.765867275
TTGTAAT51450.016.53319225-29
TAATCTC51400.016.12381430-34
GTCTCTT122850.015.801676275
CTTGTAA59400.015.55107725-29
TGTCTCT98000.015.095028270-274
AAAAAAA208100.014.95327755-59
GTAATCT54650.014.92668725-29
AGTCACC65900.014.19397520-24
ACTCCAG65650.014.02060115-19
Produced by FastQC (version 0.11.2)