FastQCFastQC Report
Wed 21 Jan 2015
smbk1_R1.trimall.1.fa

Summary

[OK]Basic Statistics

MeasureValue
Filenamesmbk1_R1.trimall.1.fa
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16948374
Sequences flagged as poor quality0
Sequence length36-282
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT119550.036.7615134
GTCTGAA127600.033.20339
CACGTCT131100.032.2529956
AGCACAC153750.028.9089682
ACGTCTG159700.026.9461827
CGTCTGA176650.024.1721558
GAGCACA189050.023.7311361
ACACGTC203650.021.907335
TCTCTTA86650.018.591438270-274
CTCTTAT95500.017.576338270-274
TCTTATA81300.017.04355270-274
CTCTAGA11200.002001687216.484919275-276
TTGTAAT49350.016.1242525-29
TAATCTC48600.015.98514930-34
ATCTAGA35050.015.802947275-276
CCTGTCT119000.015.515218275-276
CTTGTAA58500.015.02508225-29
GTAATCT51450.014.88371225-29
CCCTGTC86500.014.585501275-276
ACTCCAG61400.014.45164715-19
Produced by FastQC (version 0.11.2)