FastQCFastQC Report
Wed 21 Jan 2015
smbk1_R1.trimadapt.fa

Summary

[OK]Basic Statistics

MeasureValue
Filenamesmbk1_R1.trimadapt.fa
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20234879
Sequences flagged as poor quality0
Sequence length1-301
%GC62

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
C376070.18585235918633364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCTTT98550.0168.42937295
TCTTATT40700.0163.13232295
TATACAC265050.073.64078295
CTAGATT15750.066.026634295
CTCTTAT413750.052.878075295
CTTATAT36650.046.92679295
TCTTATA328650.044.480938290-294
TCTCTTA389050.042.75361290-294
GTCTCTT495500.039.95654295
TGTCTCT492500.039.387814295
CTTATAC379650.037.18927295
AGAGCAC139600.035.8028348
TTATACA277350.034.321712290-294
TTATACC80250.030.90103295
GGAAGAG167100.030.2622875
CTGTCTC566550.029.721464295
AAGAGCA169700.029.6424457
TCGGAAG180900.027.5824453
CTGTCTT93600.027.348309295
CCTGTCT351450.025.150988295
Produced by FastQC (version 0.11.2)