FastQCFastQC Report
Wed 21 Jan 2015
smbk1_R1.trim1.fa

Summary

[OK]Basic Statistics

MeasureValue
Filenamesmbk1_R1.trim1.fa
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17484715
Sequences flagged as poor quality0
Sequence length36-301
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT181850.0266.78168295
TATACAC110850.0229.95523295
GTCTCTT249350.0225.89078295
TGTCTCT240700.0171.66322295
TCTCTTT61200.0134.35872295
TCTTATA145500.0123.6722290-294
TCTCTTA173500.0119.57356290-294
CTTATAC159250.088.04607290-294
TTATACA116450.082.439285290-294
CTGTCTC293900.067.800896290-294
CCTGTCT191050.053.596874285-289
TCTGTCT80900.045.738434295
AGAGCAC121300.039.1808558
GCTGTCT157400.039.180847295
AAGAGCA146900.032.352887
GGAAGAG148250.032.231465
TCGGAAG160900.029.4844463
TGTGTAT70750.029.055668295
ACTGTCT72500.028.354326295
GTAGCTT30550.008740568526.915726295
Produced by FastQC (version 0.11.2)