FastQCFastQC Report
Thu 22 Jan 2015
smbk1_R1.t.p.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamesmbk1_R1.t.p.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12020342
Sequences flagged as poor quality0
Sequence length36-281
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAT22503.282912E-828.54843275
CTCTTAT57650.022.610804270-274
TCTCTTA53250.022.540176270-274
ATCTAGA21253.750426E-420.151834275
TTATACA52250.019.123348275
TCTTATA51900.018.153044270-274
GTCTCTT87150.018.016794275
TGTCTCT72550.017.183908265-269
CTTATAC70150.016.742012270-274
ACCCTGT43803.2102113E-413.035812275
TATACAC50800.012.19471270-274
CTGTCTC104300.011.399571265-269
CACATCT63254.8268703E-511.283964275
TCCCTTA23900.011.108759265-269
CCTGTCT73300.010.866276265-269
TGGCTGT77353.3062228E-510.149733275
CACCTGT85408.936222E-610.028722275
TACACAT40050.09.667466270-274
ACCTGTC108551.6245522E-69.204928275
TCTGTCT39002.4914516E-79.150137275
Produced by FastQC (version 0.11.2)