FastQCFastQC Report
Wed 21 Jan 2015
smbk1_R1.illumc.fa

Summary

[OK]Basic Statistics

MeasureValue
Filenamesmbk1_R1.illumc.fa
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20248788
Sequences flagged as poor quality0
Sequence length1-301
%GC62

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
C374040.18472216707488864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCTTT101800.0167.06612295
TCTTATT44250.0158.947295
CTAGATT18450.055.997593295
TATACAC354500.051.78669295
CTCTTAT465950.045.454857295
CTTATAT36950.039.790527295
TCTCTTA423150.038.73418290-294
TCTTATA381700.038.326874290-294
GTCTCTT532300.037.400177295
TGTCTCT528950.036.660435295
CTTATAC465150.034.854557295
AGAGCAC139250.034.2558178
CTGTCTC606250.031.068405295
GGAAGAG166550.028.751245
AAGAGCA173150.027.9412047
CTGTCTT93100.026.889532295
TCGGAAG182050.026.7063163
ATGTGTT113000.026.022299295
TCTGTCT141450.025.845043295
TTATACA363450.025.612965290-294
Produced by FastQC (version 0.11.2)