FastQCFastQC Report
Mon 30 Mar 2015
Wilkam_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWilkam_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235221536
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC115000.05.484463746-47
TGCCGTC115000.05.42274350-51
CGGTGGT56900.05.4197311
TATGCCG113650.05.36182448-49
ACACGTC403200.05.041740494-95
ACGTCTG371750.05.000842694-95
Produced by FastQC (version 0.11.2)