FastQCFastQC Report
Mon 30 Mar 2015
Ratus_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRatus_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249736489
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG422750.06.56930894-95
TCGGAAG922550.06.42878594-95
GCGTCGT450150.06.331579792-93
CGTGTAG388950.06.2461294-95
GTCGTGT456100.06.23093194-95
CGGAAGA894150.06.20651294-95
GTCGCCG54000.06.152604644-45
AGCGTCG498350.06.10210294-95
AGATCGG1132150.05.93763994-95
GAGCGTC562050.05.922556494-95
GATCGGA1049150.05.85647494-95
TAGATCG444500.05.83781394-95
GCGTCGG21850.05.817268494-95
ACGACGC11000.00279315935.6135569
ATCGGAA1074450.05.61143294-95
AAGAGCG725600.05.602309794-95
AGAGCGT649800.05.528747694-95
TCGCCGT49750.05.296243746-47
CAGATCG325000.05.143326394-95
Produced by FastQC (version 0.11.2)