FastQCFastQC Report
Mon 30 Mar 2015
Nayahu_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNayahu_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246879459
Sequences flagged as poor quality0
Sequence length101
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC99800.07.41726351
GTCGCCG44500.07.410200644-45
TCGCCGT43200.07.03432857
CCGATCT108100.06.8922712
GCGTCGT273100.06.257247492-93
ACGGCGA9958.44774E-46.2036417
CGTCGTG260400.06.196825592-93
GTCGTGT310150.05.608175894-95
AGCGTCG292800.05.592973790-91
GAGCGTC329550.05.30151790-91
CGTGTAG268850.05.10671794-95
GGTCGCC63750.05.063295
CGCCGTA76200.05.0472928
Produced by FastQC (version 0.11.2)