FastQCFastQC Report
Mon 30 Mar 2015
Nayahu_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNayahu_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246879459
Sequences flagged as poor quality0
Sequence length101
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT79250.09.96460834-35
CGTATGC96150.08.63272546-47
TGCCGTC97350.08.50261750-51
TATGCCG99600.08.28618448-49
CGTAATC116450.06.741010738-39
CTCGTAT133500.06.19969844-45
CCGTCTT143950.06.09597952-53
CCGTACG79550.05.962022332-33
ACCGTAC144850.05.76272632-33
ACACGTC328650.05.38032494-95
GCCGTCT99750.05.183304350-51
GCACACG346600.05.163024492-93
CGGTGGT48150.05.0255261
Produced by FastQC (version 0.11.2)