FastQCFastQC Report
Mon 30 Mar 2015
Nairob_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNairob_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170777073
Sequences flagged as poor quality0
Sequence length101
%GC33

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGCG7900.018.1179281
CGTTTCG16600.013.8574081
TCGCCGT31050.012.450109546-47
GTCGCCG32750.012.16639444-45
GGTCGCC45200.08.92019744-45
ACGACGC7004.2222743E-48.0328792
CGCCGTA53500.07.93499146-47
CGTTGGG55950.07.30915981
ACGCGGA9101.15282426E-47.3026341
GCCGTAT57800.07.179474448-49
GTGGTCG59050.07.14926142-43
TGGTCGC55000.06.98575542-43
CCGTATC63100.06.72676248-49
CGCGGAC9200.00103612266.6676072
CGTTGAG44600.06.64768931
CGTCGTG172050.06.48135494-95
GCGTCGT182500.06.13623194-95
CGTACCT38550.06.09973571
CGGTGGT68800.05.998246740-41
CGTTTCC120200.05.86883831
Produced by FastQC (version 0.11.2)