FastQCFastQC Report
Mon 30 Mar 2015
Nairob_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNairob_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170777073
Sequences flagged as poor quality0
Sequence length101
%GC33

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC77700.014.18263848-49
TATGCCG79350.013.82790546-47
CGTATGC82400.013.45985544-45
CTCGTAT105650.010.22789842-43
CCGTCTT111950.010.01332750-51
GCCGTCT79850.08.89316548-49
ATGCCGT88350.08.14511946-47
TCGTATG102600.06.944138542-43
TCTCGTA110800.06.30166140-41
ATCTCGT200150.06.051485540-41
CGTCTTC129100.05.83167850-51
CGTCTGA198850.05.68471516-17
AGATCGG462150.05.44730194-95
GATCGGA444700.05.42606694-95
TCGGAAG399300.05.317367694-95
CGGAAGA396100.05.270386794-95
Produced by FastQC (version 0.11.2)