FastQCFastQC Report
Mon 30 Mar 2015
Honeyb_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHoneyb_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222299610
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG22000.08.3585871
CGATGCG15401.03682396E-107.745411
TCGCCGT146250.06.73606946-47
GTCGACG9403.1565648E-46.7276912
GTCGCCG155350.06.310743344-45
CGCCGTA163150.06.03830946-47
GCCGTAT183650.05.803074448-49
GGTCGCC186350.05.630339644-45
CCGTATC195000.05.22186248-49
GTGGTCG199600.05.1970442-43
CGTATCA205250.05.180581650-51
TGGTCGC193100.05.08924342-43
Produced by FastQC (version 0.11.2)