FastQCFastQC Report
Mon 30 Mar 2015
Gengib_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGengib_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181926734
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG20300.013.6816861
CGATGCG12300.012.7271021
CGTCGTG341150.07.015391392-93
GCGTCGT357700.06.757115492-93
GTCGCGA7550.00875810356.68859342
CGTTAGT59000.06.6759881
CGTTGAT83250.06.49041561
GTCGTGT378900.06.41962894-95
AGCGTCG389200.06.28365690-91
CGTTAGA82050.06.27761271
CGTTCAT125700.06.14651731
CGCACAC93200.06.12259441
CGTTAAT93350.06.11275671
CGTTACC45850.05.94740341
CGTATAT142050.05.86564351
CGTATAC68150.05.85375071
CGTGTAG320800.05.777331494-95
CGTCAAT83050.05.7763961
CGTTATA99400.05.74070261
CGTTACA66400.05.70382451
Produced by FastQC (version 0.11.2)