FastQCFastQC Report
Mon 30 Mar 2015
Gengib_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGengib_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181926734
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGA11250.017.9579811
CCGACGC18000.013.833911
GGATCGT77600.07.2048821
ACACGTC388600.06.27128894-95
CCGGACA73600.06.06439351
CACGTCT396950.06.00924894-95
GCACACG431950.05.663542392-93
GGTCGAA53850.05.58387761
TATGCCG86000.05.487142646-47
TGCCGTC88150.05.43321548-49
CGTATGC85550.05.406123644-45
ACGTCTG348800.05.256985794-95
CACACGT471000.05.054415792-93
Produced by FastQC (version 0.11.2)